Western Blot:Article Title: Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Article Snippet: .. Antibodies used for Western blot and immunofluorescence Primary antibodies Host species Immunogen Source Incubation dilution, time, T° Anti-Giantin, polyclonal (PRB-114C), IgG1 rabbit 1-469 aa at the N-terminus of Giantin BioLegend WB: 1:5000, 20 h, 4°C IF: 1:5000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Giantin monoclonal, (ab37266), IgG1 mouse Rat Giantin Abcam IF: 1:100, 20 h, 4°C Anti-GM130, monoclonal (610822), IgG1 mouse 869-982 aa of rat GM130 BD Bioscience WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C IP: 2 μL, 15 h, 4°C Anti-GMAP210, monoclonal, (611712), IgG1 mouse 159-365 aa of human GMAP210 BD Transduction Laboratories WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C Anti-PDIA1, monoclonal (1D3), IgG1 mouse Peptide mapped at the end Cterminus of rat PDIA1 Enzo Life Sciences IF: 1:300, 20 h, 4°C Anti-TGN46, polyclonal (AHP500), IgG1 sheep Recombinant human TGN46 AbDSerotec IF: 1:250, 20 h, 4°C Anti-PAPST1, polyclonal, IgG rabbit KAVPTEPPVQKV sequence of mouse PAPST1 Dr. Shoko Nishihara IF: 1:500, 20 h, 4°C Anti-GOLPH3, polyclonal (ab98023), IgG rabbit 1-100 aa of human GOLPH3 Abcam IF: 1:50, 20 h, 4°C Anti-MUC1, monoclonal (M8), IgG1 mouse DTR epitope of MUC1 VNTR Dr. Dallas Swallow IF: 1:50, 20 h, 4°C Anti-MUC7, monoclonal (PANH3), IgG1 mouse CRPKLPPSPNKP PKFPNPHQP sequence of MUC7 Nterminus Dr. Ulla Mandel IF: 1:50, 20 h, 4°C Anti-Gal3ST2, polyclonal (orb183825), IgG rabbit KLH-conjugated peptide (33178 aa) of human Gal3ST2 Biorbyt IF: 1:50, 20 h, 4°C Anti-Gal3ST2, (H00079690-B01), IgG, polyclonal rabbit KLH-conjugated CELGPRRLRGEV ERL peptide GenScript WB: 1:1000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Gal3ST4, polyclonal rabbit KLH-conjugated peptide (411- 476 aa) of human Gal3ST4 Biorbyt WB: 1:1000, 20 h, 4°C IF: 1:25, 20 h, 4°C IP: 4 μL, 15 h, 4°C Anti-GalNAcT1, monoclonal (4D8), IgG1 mouse 41-559 aa of GalNAcT1 Dra. .. Ulla Mandel WB: 1:10, 20 h, 4°C Anti-Core2 GlcNAc-T2, polyclonal (ab227972), IgG rabbit Recombinant fragment of Core2 GlcNAcT2 Abcam IP: 2 μL, 15 h, 4°C Anti-Integrin α6, monoclonal (BQ16), IgG, sc-13542 mouse Human Integrin α6 Santa Cruz Biotechnology IP: 1 μL, 15 h, 4°C Anti-MUC5B, monoclonal (clone PANH2), IgG1 mouse Partially deglycosylated MUC5B Dra.
Article Title:
Article Snippet: .. Primary antibodies: anti-synaptophysin (101 002, Synaptic Systems) anti-synaptophysin (101 011, Synaptic Systems) anti-synaptophysin (101 308, Synaptic Systems) 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation anti-VAMP2 (104 211, Synaptic Systems) anti-Rab3A (107 111, Synaptic Systems) anti-ATG9A (ab108338, Abcam) anti-LC3 (PM036, MBL) anti-AP4E1 (612018, BD Transduction LaboratoriesTM) anti-Actin (691002, MP Biomedicals) anti-HA (MMS-101R, Covance) anti-GM130 (610822, BD Bioscience) anti-TGN46 (610822, AHP500GT, AbD Serotec) anti-TOMM40 (18409-1-AP, Proteintech) Materials for IF: Alexa Fluor 488 anti-mouse IgG (H+L) (A11001, Invitrogen) Alexa Fluor 594 anti-mouse IgG (H+L) (A21203, Invitrogen) Alexa Fluor 647 anti-mouse IgG (H+L) (A32787, Invitrogen) Alexa Fluor 594 anti-rabbit IgG (H+L) (A11037, Invitrogen) Alexa Fluor 647 anti-sheep IgG (H+L) (A21448, Invitrogen) Alexa Fluor 488 anti-guinea pig IgG (H+L) (A11073, Invitrogen) Streptavidin, Alexa Fluor 647 Conjugate (S32357, Invitrogen) Materials for WB: IRDye 800CW anti-mouse (926-32210, LI-COR) IRDye 800CW anti-rabbit (926-32211, LI-COR) IRDye 800CW anti-guinea pig (925-32411, LI-COR) IRDye 680LT anti-mouse (926-68020, LI-COR) IRDye 680LT anti-rabbit (926-68021, LI-COR) IRDye 680RD Streptavidin (926-68079, LI-COR) anti-synaptophysin (101 002): Suitable for WB, IP, ICC, IHC, EM, ELISA; reacts with Human, Rat, Mouse, Hamster, Cow, Chicken, Frog, Zebrafish [https://sysy.com/product/101002] anti-synaptophysin (101 011): Suitable for WB, IP, ICC, IHC, IHC-P, EM, ELISA; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates. .. Other species not tested yet); KO validated PubMed: 31940485 [https://sysy.com/ product/101011] anti-synaptophysin (101 308): Suitable for WB, IP, ICC, IHC, IHC-P; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates.
Article Title: The DNA Damage Response kinase ATM restricts Golgi extension
Article Snippet: .. siControl was the universal negative control siRNA #1 (SIC001, Sigma-Aldrich) siATM (SASI_Hs01_00093615, 5’ -CUUAGCAGGAGGUGUAAAU [dT][dT]-3’, Sigma-Aldrich) siGOLPH3 (SASI_Hs01_00133693, 5’-GGUGUAUUGACAACAGAGA[dT][dT]-3’, Sigma-Aldrich) Primary antibodies : Anti-PI4P (Z-P004, Echelon Biosciences, IF at 1/100); anti-GOLPH3 (SAB4200341, Sigma-Aldrich, IF at 1/300, WB at 1/2,000); anti-TGN46 (AHP500G, Bio-Rad, IF at 1/300, WB at 1/2,000); anti-GM130 (HPA021799, Sigma-Aldrich, IF at 1/300); anti-P-Ser1981-ATM (4526, Cell Signaling, IF at 1/200); anti-ATM (GTX132147, CliniSciences, WB at 1/2,000); anti-SQ/TQ-P (2851S, Ozyme, IF at 1/500); anti-PGK1 (13472727, ThermoFisher, WB at 1/1,000); anti-Calnexin (610523, Becton Dickinson, WB at 1/1,000); anti- Y H2AX (05-636, Millipore, IF at 1/500). .. Secondary antibodies for WB: anti-rabbit (10733944, Fisher Scientific, WB at 1/15,000); anti-mouse (A9044, Sigma-Aldrich, WB at 1/5,000); anti-sheep (A3415, Merck, WB at 1/2,500).
Immunofluorescence:Article Title: Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Article Snippet: .. Antibodies used for Western blot and immunofluorescence Primary antibodies Host species Immunogen Source Incubation dilution, time, T° Anti-Giantin, polyclonal (PRB-114C), IgG1 rabbit 1-469 aa at the N-terminus of Giantin BioLegend WB: 1:5000, 20 h, 4°C IF: 1:5000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Giantin monoclonal, (ab37266), IgG1 mouse Rat Giantin Abcam IF: 1:100, 20 h, 4°C Anti-GM130, monoclonal (610822), IgG1 mouse 869-982 aa of rat GM130 BD Bioscience WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C IP: 2 μL, 15 h, 4°C Anti-GMAP210, monoclonal, (611712), IgG1 mouse 159-365 aa of human GMAP210 BD Transduction Laboratories WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C Anti-PDIA1, monoclonal (1D3), IgG1 mouse Peptide mapped at the end Cterminus of rat PDIA1 Enzo Life Sciences IF: 1:300, 20 h, 4°C Anti-TGN46, polyclonal (AHP500), IgG1 sheep Recombinant human TGN46 AbDSerotec IF: 1:250, 20 h, 4°C Anti-PAPST1, polyclonal, IgG rabbit KAVPTEPPVQKV sequence of mouse PAPST1 Dr. Shoko Nishihara IF: 1:500, 20 h, 4°C Anti-GOLPH3, polyclonal (ab98023), IgG rabbit 1-100 aa of human GOLPH3 Abcam IF: 1:50, 20 h, 4°C Anti-MUC1, monoclonal (M8), IgG1 mouse DTR epitope of MUC1 VNTR Dr. Dallas Swallow IF: 1:50, 20 h, 4°C Anti-MUC7, monoclonal (PANH3), IgG1 mouse CRPKLPPSPNKP PKFPNPHQP sequence of MUC7 Nterminus Dr. Ulla Mandel IF: 1:50, 20 h, 4°C Anti-Gal3ST2, polyclonal (orb183825), IgG rabbit KLH-conjugated peptide (33178 aa) of human Gal3ST2 Biorbyt IF: 1:50, 20 h, 4°C Anti-Gal3ST2, (H00079690-B01), IgG, polyclonal rabbit KLH-conjugated CELGPRRLRGEV ERL peptide GenScript WB: 1:1000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Gal3ST4, polyclonal rabbit KLH-conjugated peptide (411- 476 aa) of human Gal3ST4 Biorbyt WB: 1:1000, 20 h, 4°C IF: 1:25, 20 h, 4°C IP: 4 μL, 15 h, 4°C Anti-GalNAcT1, monoclonal (4D8), IgG1 mouse 41-559 aa of GalNAcT1 Dra. .. Ulla Mandel WB: 1:10, 20 h, 4°C Anti-Core2 GlcNAc-T2, polyclonal (ab227972), IgG rabbit Recombinant fragment of Core2 GlcNAcT2 Abcam IP: 2 μL, 15 h, 4°C Anti-Integrin α6, monoclonal (BQ16), IgG, sc-13542 mouse Human Integrin α6 Santa Cruz Biotechnology IP: 1 μL, 15 h, 4°C Anti-MUC5B, monoclonal (clone PANH2), IgG1 mouse Partially deglycosylated MUC5B Dra.
Incubation:Article Title: Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Article Snippet: .. Antibodies used for Western blot and immunofluorescence Primary antibodies Host species Immunogen Source Incubation dilution, time, T° Anti-Giantin, polyclonal (PRB-114C), IgG1 rabbit 1-469 aa at the N-terminus of Giantin BioLegend WB: 1:5000, 20 h, 4°C IF: 1:5000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Giantin monoclonal, (ab37266), IgG1 mouse Rat Giantin Abcam IF: 1:100, 20 h, 4°C Anti-GM130, monoclonal (610822), IgG1 mouse 869-982 aa of rat GM130 BD Bioscience WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C IP: 2 μL, 15 h, 4°C Anti-GMAP210, monoclonal, (611712), IgG1 mouse 159-365 aa of human GMAP210 BD Transduction Laboratories WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C Anti-PDIA1, monoclonal (1D3), IgG1 mouse Peptide mapped at the end Cterminus of rat PDIA1 Enzo Life Sciences IF: 1:300, 20 h, 4°C Anti-TGN46, polyclonal (AHP500), IgG1 sheep Recombinant human TGN46 AbDSerotec IF: 1:250, 20 h, 4°C Anti-PAPST1, polyclonal, IgG rabbit KAVPTEPPVQKV sequence of mouse PAPST1 Dr. Shoko Nishihara IF: 1:500, 20 h, 4°C Anti-GOLPH3, polyclonal (ab98023), IgG rabbit 1-100 aa of human GOLPH3 Abcam IF: 1:50, 20 h, 4°C Anti-MUC1, monoclonal (M8), IgG1 mouse DTR epitope of MUC1 VNTR Dr. Dallas Swallow IF: 1:50, 20 h, 4°C Anti-MUC7, monoclonal (PANH3), IgG1 mouse CRPKLPPSPNKP PKFPNPHQP sequence of MUC7 Nterminus Dr. Ulla Mandel IF: 1:50, 20 h, 4°C Anti-Gal3ST2, polyclonal (orb183825), IgG rabbit KLH-conjugated peptide (33178 aa) of human Gal3ST2 Biorbyt IF: 1:50, 20 h, 4°C Anti-Gal3ST2, (H00079690-B01), IgG, polyclonal rabbit KLH-conjugated CELGPRRLRGEV ERL peptide GenScript WB: 1:1000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Gal3ST4, polyclonal rabbit KLH-conjugated peptide (411- 476 aa) of human Gal3ST4 Biorbyt WB: 1:1000, 20 h, 4°C IF: 1:25, 20 h, 4°C IP: 4 μL, 15 h, 4°C Anti-GalNAcT1, monoclonal (4D8), IgG1 mouse 41-559 aa of GalNAcT1 Dra. .. Ulla Mandel WB: 1:10, 20 h, 4°C Anti-Core2 GlcNAc-T2, polyclonal (ab227972), IgG rabbit Recombinant fragment of Core2 GlcNAcT2 Abcam IP: 2 μL, 15 h, 4°C Anti-Integrin α6, monoclonal (BQ16), IgG, sc-13542 mouse Human Integrin α6 Santa Cruz Biotechnology IP: 1 μL, 15 h, 4°C Anti-MUC5B, monoclonal (clone PANH2), IgG1 mouse Partially deglycosylated MUC5B Dra.
Article Title: Protein-based tools for the detection and characterisation of Oropouche virus infection
Article Snippet: .. Permeabilised cells were incubated with primary antibodies and anti-OROV nanobodies at 37°C for 30 min, diluted as follows: mouse anti-OROV antiserum, 1:500 [ ]; AlexaFluor568 tagged anti-OROV nanobodies, 1:4000; anti-TGN46 (Bio-Rad AHP500), 1:500. .. After washing, secondary antibodies were applied at 37°C for 30 min, diluted as follows: AlexaFluor488 anti-mouse and AlexaFluor647 anti-sheep (Thermo Fisher Scientific), 1:1000.
Article Title: Protein-based tools for the detection and characterisation of Oropouche virus infection
Article Snippet: .. Permeabilised cells were incubated with primary antibodies and anti-OROV nanobodies at 37 °C for 30 min, diluted as follows: mouse anti-OROV antiserum, 1:500 (Concha et al, ); AlexaFluor568 tagged anti-OROV nanobodies, 1:4000; anti-TGN46 (Bio-Rad AHP500), 1:500. .. After washing, secondary antibodies were applied at 37 °C for 30 min, diluted as follows: AlexaFluor488 anti-mouse and AlexaFluor647 anti-sheep (Thermo Fisher Scientific), 1:1000.
Recombinant:Article Title: Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Article Snippet: .. Antibodies used for Western blot and immunofluorescence Primary antibodies Host species Immunogen Source Incubation dilution, time, T° Anti-Giantin, polyclonal (PRB-114C), IgG1 rabbit 1-469 aa at the N-terminus of Giantin BioLegend WB: 1:5000, 20 h, 4°C IF: 1:5000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Giantin monoclonal, (ab37266), IgG1 mouse Rat Giantin Abcam IF: 1:100, 20 h, 4°C Anti-GM130, monoclonal (610822), IgG1 mouse 869-982 aa of rat GM130 BD Bioscience WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C IP: 2 μL, 15 h, 4°C Anti-GMAP210, monoclonal, (611712), IgG1 mouse 159-365 aa of human GMAP210 BD Transduction Laboratories WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C Anti-PDIA1, monoclonal (1D3), IgG1 mouse Peptide mapped at the end Cterminus of rat PDIA1 Enzo Life Sciences IF: 1:300, 20 h, 4°C Anti-TGN46, polyclonal (AHP500), IgG1 sheep Recombinant human TGN46 AbDSerotec IF: 1:250, 20 h, 4°C Anti-PAPST1, polyclonal, IgG rabbit KAVPTEPPVQKV sequence of mouse PAPST1 Dr. Shoko Nishihara IF: 1:500, 20 h, 4°C Anti-GOLPH3, polyclonal (ab98023), IgG rabbit 1-100 aa of human GOLPH3 Abcam IF: 1:50, 20 h, 4°C Anti-MUC1, monoclonal (M8), IgG1 mouse DTR epitope of MUC1 VNTR Dr. Dallas Swallow IF: 1:50, 20 h, 4°C Anti-MUC7, monoclonal (PANH3), IgG1 mouse CRPKLPPSPNKP PKFPNPHQP sequence of MUC7 Nterminus Dr. Ulla Mandel IF: 1:50, 20 h, 4°C Anti-Gal3ST2, polyclonal (orb183825), IgG rabbit KLH-conjugated peptide (33178 aa) of human Gal3ST2 Biorbyt IF: 1:50, 20 h, 4°C Anti-Gal3ST2, (H00079690-B01), IgG, polyclonal rabbit KLH-conjugated CELGPRRLRGEV ERL peptide GenScript WB: 1:1000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Gal3ST4, polyclonal rabbit KLH-conjugated peptide (411- 476 aa) of human Gal3ST4 Biorbyt WB: 1:1000, 20 h, 4°C IF: 1:25, 20 h, 4°C IP: 4 μL, 15 h, 4°C Anti-GalNAcT1, monoclonal (4D8), IgG1 mouse 41-559 aa of GalNAcT1 Dra. .. Ulla Mandel WB: 1:10, 20 h, 4°C Anti-Core2 GlcNAc-T2, polyclonal (ab227972), IgG rabbit Recombinant fragment of Core2 GlcNAcT2 Abcam IP: 2 μL, 15 h, 4°C Anti-Integrin α6, monoclonal (BQ16), IgG, sc-13542 mouse Human Integrin α6 Santa Cruz Biotechnology IP: 1 μL, 15 h, 4°C Anti-MUC5B, monoclonal (clone PANH2), IgG1 mouse Partially deglycosylated MUC5B Dra.
Sequencing:Article Title: Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sjögren’s disease
Article Snippet: .. Antibodies used for Western blot and immunofluorescence Primary antibodies Host species Immunogen Source Incubation dilution, time, T° Anti-Giantin, polyclonal (PRB-114C), IgG1 rabbit 1-469 aa at the N-terminus of Giantin BioLegend WB: 1:5000, 20 h, 4°C IF: 1:5000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Giantin monoclonal, (ab37266), IgG1 mouse Rat Giantin Abcam IF: 1:100, 20 h, 4°C Anti-GM130, monoclonal (610822), IgG1 mouse 869-982 aa of rat GM130 BD Bioscience WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C IP: 2 μL, 15 h, 4°C Anti-GMAP210, monoclonal, (611712), IgG1 mouse 159-365 aa of human GMAP210 BD Transduction Laboratories WB: 1:1000, 20 h, 4°C IF: 1:50, 20 h, 4°C Anti-PDIA1, monoclonal (1D3), IgG1 mouse Peptide mapped at the end Cterminus of rat PDIA1 Enzo Life Sciences IF: 1:300, 20 h, 4°C Anti-TGN46, polyclonal (AHP500), IgG1 sheep Recombinant human TGN46 AbDSerotec IF: 1:250, 20 h, 4°C Anti-PAPST1, polyclonal, IgG rabbit KAVPTEPPVQKV sequence of mouse PAPST1 Dr. Shoko Nishihara IF: 1:500, 20 h, 4°C Anti-GOLPH3, polyclonal (ab98023), IgG rabbit 1-100 aa of human GOLPH3 Abcam IF: 1:50, 20 h, 4°C Anti-MUC1, monoclonal (M8), IgG1 mouse DTR epitope of MUC1 VNTR Dr. Dallas Swallow IF: 1:50, 20 h, 4°C Anti-MUC7, monoclonal (PANH3), IgG1 mouse CRPKLPPSPNKP PKFPNPHQP sequence of MUC7 Nterminus Dr. Ulla Mandel IF: 1:50, 20 h, 4°C Anti-Gal3ST2, polyclonal (orb183825), IgG rabbit KLH-conjugated peptide (33178 aa) of human Gal3ST2 Biorbyt IF: 1:50, 20 h, 4°C Anti-Gal3ST2, (H00079690-B01), IgG, polyclonal rabbit KLH-conjugated CELGPRRLRGEV ERL peptide GenScript WB: 1:1000, 20 h, 4°C IP: 1 μL, 15 h, 4°C Anti-Gal3ST4, polyclonal rabbit KLH-conjugated peptide (411- 476 aa) of human Gal3ST4 Biorbyt WB: 1:1000, 20 h, 4°C IF: 1:25, 20 h, 4°C IP: 4 μL, 15 h, 4°C Anti-GalNAcT1, monoclonal (4D8), IgG1 mouse 41-559 aa of GalNAcT1 Dra. .. Ulla Mandel WB: 1:10, 20 h, 4°C Anti-Core2 GlcNAc-T2, polyclonal (ab227972), IgG rabbit Recombinant fragment of Core2 GlcNAcT2 Abcam IP: 2 μL, 15 h, 4°C Anti-Integrin α6, monoclonal (BQ16), IgG, sc-13542 mouse Human Integrin α6 Santa Cruz Biotechnology IP: 1 μL, 15 h, 4°C Anti-MUC5B, monoclonal (clone PANH2), IgG1 mouse Partially deglycosylated MUC5B Dra.
Biomarker Discovery:Article Title:
Article Snippet: .. Primary antibodies: anti-synaptophysin (101 002, Synaptic Systems) anti-synaptophysin (101 011, Synaptic Systems) anti-synaptophysin (101 308, Synaptic Systems) 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation anti-VAMP2 (104 211, Synaptic Systems) anti-Rab3A (107 111, Synaptic Systems) anti-ATG9A (ab108338, Abcam) anti-LC3 (PM036, MBL) anti-AP4E1 (612018, BD Transduction LaboratoriesTM) anti-Actin (691002, MP Biomedicals) anti-HA (MMS-101R, Covance) anti-GM130 (610822, BD Bioscience) anti-TGN46 (610822, AHP500GT, AbD Serotec) anti-TOMM40 (18409-1-AP, Proteintech) Materials for IF: Alexa Fluor 488 anti-mouse IgG (H+L) (A11001, Invitrogen) Alexa Fluor 594 anti-mouse IgG (H+L) (A21203, Invitrogen) Alexa Fluor 647 anti-mouse IgG (H+L) (A32787, Invitrogen) Alexa Fluor 594 anti-rabbit IgG (H+L) (A11037, Invitrogen) Alexa Fluor 647 anti-sheep IgG (H+L) (A21448, Invitrogen) Alexa Fluor 488 anti-guinea pig IgG (H+L) (A11073, Invitrogen) Streptavidin, Alexa Fluor 647 Conjugate (S32357, Invitrogen) Materials for WB: IRDye 800CW anti-mouse (926-32210, LI-COR) IRDye 800CW anti-rabbit (926-32211, LI-COR) IRDye 800CW anti-guinea pig (925-32411, LI-COR) IRDye 680LT anti-mouse (926-68020, LI-COR) IRDye 680LT anti-rabbit (926-68021, LI-COR) IRDye 680RD Streptavidin (926-68079, LI-COR) anti-synaptophysin (101 002): Suitable for WB, IP, ICC, IHC, EM, ELISA; reacts with Human, Rat, Mouse, Hamster, Cow, Chicken, Frog, Zebrafish [https://sysy.com/product/101002] anti-synaptophysin (101 011): Suitable for WB, IP, ICC, IHC, IHC-P, EM, ELISA; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates. .. Other species not tested yet); KO validated PubMed: 31940485 [https://sysy.com/ product/101011] anti-synaptophysin (101 308): Suitable for WB, IP, ICC, IHC, IHC-P; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates.
Immunocytochemistry:Article Title:
Article Snippet: .. Primary antibodies: anti-synaptophysin (101 002, Synaptic Systems) anti-synaptophysin (101 011, Synaptic Systems) anti-synaptophysin (101 308, Synaptic Systems) 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation anti-VAMP2 (104 211, Synaptic Systems) anti-Rab3A (107 111, Synaptic Systems) anti-ATG9A (ab108338, Abcam) anti-LC3 (PM036, MBL) anti-AP4E1 (612018, BD Transduction LaboratoriesTM) anti-Actin (691002, MP Biomedicals) anti-HA (MMS-101R, Covance) anti-GM130 (610822, BD Bioscience) anti-TGN46 (610822, AHP500GT, AbD Serotec) anti-TOMM40 (18409-1-AP, Proteintech) Materials for IF: Alexa Fluor 488 anti-mouse IgG (H+L) (A11001, Invitrogen) Alexa Fluor 594 anti-mouse IgG (H+L) (A21203, Invitrogen) Alexa Fluor 647 anti-mouse IgG (H+L) (A32787, Invitrogen) Alexa Fluor 594 anti-rabbit IgG (H+L) (A11037, Invitrogen) Alexa Fluor 647 anti-sheep IgG (H+L) (A21448, Invitrogen) Alexa Fluor 488 anti-guinea pig IgG (H+L) (A11073, Invitrogen) Streptavidin, Alexa Fluor 647 Conjugate (S32357, Invitrogen) Materials for WB: IRDye 800CW anti-mouse (926-32210, LI-COR) IRDye 800CW anti-rabbit (926-32211, LI-COR) IRDye 800CW anti-guinea pig (925-32411, LI-COR) IRDye 680LT anti-mouse (926-68020, LI-COR) IRDye 680LT anti-rabbit (926-68021, LI-COR) IRDye 680RD Streptavidin (926-68079, LI-COR) anti-synaptophysin (101 002): Suitable for WB, IP, ICC, IHC, EM, ELISA; reacts with Human, Rat, Mouse, Hamster, Cow, Chicken, Frog, Zebrafish [https://sysy.com/product/101002] anti-synaptophysin (101 011): Suitable for WB, IP, ICC, IHC, IHC-P, EM, ELISA; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates. .. Other species not tested yet); KO validated PubMed: 31940485 [https://sysy.com/ product/101011] anti-synaptophysin (101 308): Suitable for WB, IP, ICC, IHC, IHC-P; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates.
Immunohistochemistry:Article Title:
Article Snippet: .. Primary antibodies: anti-synaptophysin (101 002, Synaptic Systems) anti-synaptophysin (101 011, Synaptic Systems) anti-synaptophysin (101 308, Synaptic Systems) 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation anti-VAMP2 (104 211, Synaptic Systems) anti-Rab3A (107 111, Synaptic Systems) anti-ATG9A (ab108338, Abcam) anti-LC3 (PM036, MBL) anti-AP4E1 (612018, BD Transduction LaboratoriesTM) anti-Actin (691002, MP Biomedicals) anti-HA (MMS-101R, Covance) anti-GM130 (610822, BD Bioscience) anti-TGN46 (610822, AHP500GT, AbD Serotec) anti-TOMM40 (18409-1-AP, Proteintech) Materials for IF: Alexa Fluor 488 anti-mouse IgG (H+L) (A11001, Invitrogen) Alexa Fluor 594 anti-mouse IgG (H+L) (A21203, Invitrogen) Alexa Fluor 647 anti-mouse IgG (H+L) (A32787, Invitrogen) Alexa Fluor 594 anti-rabbit IgG (H+L) (A11037, Invitrogen) Alexa Fluor 647 anti-sheep IgG (H+L) (A21448, Invitrogen) Alexa Fluor 488 anti-guinea pig IgG (H+L) (A11073, Invitrogen) Streptavidin, Alexa Fluor 647 Conjugate (S32357, Invitrogen) Materials for WB: IRDye 800CW anti-mouse (926-32210, LI-COR) IRDye 800CW anti-rabbit (926-32211, LI-COR) IRDye 800CW anti-guinea pig (925-32411, LI-COR) IRDye 680LT anti-mouse (926-68020, LI-COR) IRDye 680LT anti-rabbit (926-68021, LI-COR) IRDye 680RD Streptavidin (926-68079, LI-COR) anti-synaptophysin (101 002): Suitable for WB, IP, ICC, IHC, EM, ELISA; reacts with Human, Rat, Mouse, Hamster, Cow, Chicken, Frog, Zebrafish [https://sysy.com/product/101002] anti-synaptophysin (101 011): Suitable for WB, IP, ICC, IHC, IHC-P, EM, ELISA; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates. .. Other species not tested yet); KO validated PubMed: 31940485 [https://sysy.com/ product/101011] anti-synaptophysin (101 308): Suitable for WB, IP, ICC, IHC, IHC-P; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates.
Enzyme-linked Immunosorbent Assay:Article Title:
Article Snippet: .. Primary antibodies: anti-synaptophysin (101 002, Synaptic Systems) anti-synaptophysin (101 011, Synaptic Systems) anti-synaptophysin (101 308, Synaptic Systems) 3 n atu re p o rtfo lio | rep o rtin g su m m ary M a rch 2021 Validation anti-VAMP2 (104 211, Synaptic Systems) anti-Rab3A (107 111, Synaptic Systems) anti-ATG9A (ab108338, Abcam) anti-LC3 (PM036, MBL) anti-AP4E1 (612018, BD Transduction LaboratoriesTM) anti-Actin (691002, MP Biomedicals) anti-HA (MMS-101R, Covance) anti-GM130 (610822, BD Bioscience) anti-TGN46 (610822, AHP500GT, AbD Serotec) anti-TOMM40 (18409-1-AP, Proteintech) Materials for IF: Alexa Fluor 488 anti-mouse IgG (H+L) (A11001, Invitrogen) Alexa Fluor 594 anti-mouse IgG (H+L) (A21203, Invitrogen) Alexa Fluor 647 anti-mouse IgG (H+L) (A32787, Invitrogen) Alexa Fluor 594 anti-rabbit IgG (H+L) (A11037, Invitrogen) Alexa Fluor 647 anti-sheep IgG (H+L) (A21448, Invitrogen) Alexa Fluor 488 anti-guinea pig IgG (H+L) (A11073, Invitrogen) Streptavidin, Alexa Fluor 647 Conjugate (S32357, Invitrogen) Materials for WB: IRDye 800CW anti-mouse (926-32210, LI-COR) IRDye 800CW anti-rabbit (926-32211, LI-COR) IRDye 800CW anti-guinea pig (925-32411, LI-COR) IRDye 680LT anti-mouse (926-68020, LI-COR) IRDye 680LT anti-rabbit (926-68021, LI-COR) IRDye 680RD Streptavidin (926-68079, LI-COR) anti-synaptophysin (101 002): Suitable for WB, IP, ICC, IHC, EM, ELISA; reacts with Human, Rat, Mouse, Hamster, Cow, Chicken, Frog, Zebrafish [https://sysy.com/product/101002] anti-synaptophysin (101 011): Suitable for WB, IP, ICC, IHC, IHC-P, EM, ELISA; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates. .. Other species not tested yet); KO validated PubMed: 31940485 [https://sysy.com/ product/101011] anti-synaptophysin (101 308): Suitable for WB, IP, ICC, IHC, IHC-P; reacts with Human, Rat, Mouse, Mammals (weak signals for Zebrafish and other vertebrates.
Negative Control:Article Title: The DNA Damage Response kinase ATM restricts Golgi extension
Article Snippet: .. siControl was the universal negative control siRNA #1 (SIC001, Sigma-Aldrich) siATM (SASI_Hs01_00093615, 5’ -CUUAGCAGGAGGUGUAAAU [dT][dT]-3’, Sigma-Aldrich) siGOLPH3 (SASI_Hs01_00133693, 5’-GGUGUAUUGACAACAGAGA[dT][dT]-3’, Sigma-Aldrich) Primary antibodies : Anti-PI4P (Z-P004, Echelon Biosciences, IF at 1/100); anti-GOLPH3 (SAB4200341, Sigma-Aldrich, IF at 1/300, WB at 1/2,000); anti-TGN46 (AHP500G, Bio-Rad, IF at 1/300, WB at 1/2,000); anti-GM130 (HPA021799, Sigma-Aldrich, IF at 1/300); anti-P-Ser1981-ATM (4526, Cell Signaling, IF at 1/200); anti-ATM (GTX132147, CliniSciences, WB at 1/2,000); anti-SQ/TQ-P (2851S, Ozyme, IF at 1/500); anti-PGK1 (13472727, ThermoFisher, WB at 1/1,000); anti-Calnexin (610523, Becton Dickinson, WB at 1/1,000); anti- Y H2AX (05-636, Millipore, IF at 1/500). .. Secondary antibodies for WB: anti-rabbit (10733944, Fisher Scientific, WB at 1/15,000); anti-mouse (A9044, Sigma-Aldrich, WB at 1/5,000); anti-sheep (A3415, Merck, WB at 1/2,500).
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